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Researchers reap scientific rewards from Raman spectroscopy to laser for external bioprospecting

Time2024-01-15 09:53:23 visits:365

We investigated the potential of laser selection over a broad optical range from UV to visible to infrared (excitation wavelengths 325, 532, 785 and 1064 nm) for combinatorial analysis of Earth-associated extremophiles (Caenorhabditis elegans, C. nematodes and ring green algae), carbohydrate molecules, and Martian and lunar surface regolith simulants as simulated mineral mixtures (P-MRS, S-MRS, LRS and JSC-1).

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We show that optimization of the laser photon energy provides (at least one selected excitation wavelength) high-end quality Raman spectra for each sample examined. In most cases, the infrared spectral range is advanced for biological samples, while excitation in the visible and UV spectral ranges is often advantageous, or at least sufficient to accurately identify/resolve minerals under the luminescent laser spot on planetary surface simulants Mutually.

UV excitation does not always provide significant contrast in the Raman Stokes response to induced photoluminescence in the biomolecules under study. The most prominent features in the Raman spectra of biological samples are assigned to their specific pigments and are also considered biomolecular signatures of extremophiles. The key question of the specific advantages and limitations of each particular excitation source implies research into obtaining scientific returns from Raman spectroscopy for external bioprospecting, e.g., single excitation wavelengths or the best between dual excitation wavelengths for biological and geological spectroscopic data. Good deal.

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